Fundamental knowledge about cell – surface interactions can be applied in the development of wound dressings and scaffolds to encourage chronic wounds to heal. These dressings may then encourage wound healing through enhanced cell migration, control of collagen deposition and enhanced angiogenesis. Using plasma polymerisation, a solvent free technique that allows for the deposition of thin, conformable coatings onto a variety of substrates both 2D and 3D, we aim to develop dressings which are capable of directing cell behaviour within the healing wound. Initial in vitro assays determined that the application of a plasma polymer from a monomer with acid functionality encourages keratinocyte adhesion, proliferation and migration, whilst surfaces produced using an amine functionalised monomer enhanced fibroblast proliferation and migration. To test the effect of these surfaces in vivo, standard care wound dressings were plasma coated with either acrylic acid or allylamine and applied to 6mm excisional wounds on the backs of mice. At day 3, a significant increase in the rate of wound healing was observed in mice treated with dressings plasma coated with allylamine compared to uncoated dressings, with a significantly reduced wound area. These results suggest that plasma polymerisation may allow for the development of new dressings which can enhance wound closure by directing cell behaviour.